Development and validation of a simple solid-phase extraction method coupled with liquid chromatography-triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

文献类型： 外文期刊

第一作者： Zheng, Weijia

作者： Zheng, Weijia;Park, Jin-A;Abd El-Aty, A. M.;Kim, Seong-Kwan;Cho, Sang-Hyun;Choi, Jeong-Min;Shin, Ho-Chul;Abd El-Aty, A. M.;Warda, Mohamad;Wang, Jing;Shim, Jae-Han

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关键词： antibiotics; lincomycin; royal jelly; solid-phase extraction; tandem mass spectrometry; tylosin A and B

期刊名称：BIOMEDICAL CHROMATOGRAPHY （ 影响因子：1.902； 五年影响因子：1.809 ）

ISSN： 0269-3879

年卷期： 2018 年 32 卷 4 期

页码：

收录情况： SCI

摘要： We have developed an analytical method for the determination of lincomycin, tylosin A and tylosin B residues in royal jelly using liquid chromatography-triple quadrupole tandem mass spectrometry analysis. For extraction and purification, we employed 1% trifluoroacetic acid and 0.1m Na(2)EDTA solutions along with an Oasis HLB cartridge. The target antibiotics were well separated in a Kinetex EVO C-18 reversed-phase analytical column using a combination of 0.1% formate acid in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (5-50g/kg) in matrix-matched standard calibration. The coefficients of determination (R-2) were 0.9933, 0.9933 and 0.996, for tylosin A, tylosin B and lincomycin, respectively. Fortified royal jelly spiked with three different concentrations of the tested antibiotics (5, 10 and 20g/kg) yielded recoveries in the range 80.94-109.26% with relative standard deviations 4%. The proposed method was applied to monitor 11 brand of royal jelly collected from domestic markets and an imported brand from New Zealand; all the samples tested negative for lincomycin, tylosin A and tylosin B residues. In conclusion, 1% trifluoroacetic acid and 0.1m Na(2)EDTA aqueous solvents combined with solid-phase extraction could effectively complete the sample preparation process for royal jelly before analysis. The developed approach can be applied for a routine analysis of lincomycin, tylosin A and tylosin B residues in royal jelly.

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