Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Zhang, Gaiping;Liu, Chang;Liu, Yunchao;Feng, Hua;Chen, Yumei;Wang, Yinbiao;Deng, Ruiguang;Zhang, Gaiping;Chen, Hongying;Zhang, Gaiping;Wang, Juan;Liu, Dongmin

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2018 年 163 卷 4 期

页码：

收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) is an economically important pathogen in domestic pigs and wild boars all around the world. To understand the molecular epidemiology of PCV2 strains circulating in central China and to provide a potential vaccine candidate strain, we analyzed the genetic variations of 46 PCV2 isolates circulating from 2009 to 2016 in Henan Province (24 detected in the field from 2009-2013 and 22 from 2013-2016) and evaluated the efficacy of an isolate as a vaccine candidate strain in a mouse model. We found that PCV2b was the predominant genotype and PCV2b-1C was the main subtype. The PCV2 isolate DF-1, which had a virus titer of 10(6.5) TCID50/mL and a stable genome, was selected and used to immunize Kunming mice. Enzyme-linked immunosorbent assay (ELISA), immunoperoxidase monolayer assay (IPMA), and virus neutralization test (VNT) results indicated that the DF-1 vaccine candidate strain could elicit a level of specific antibodies and neutralizing antibodies similar to those induced by a commercial vaccine. Polymerase chain reaction (PCR) detection of virus in vaccinated mice after challenge revealed that DF-1 vaccination was effective in clearing the virus in different tissues. Hence, the PCV2 isolate DF-1, a circulating subtype of PCV2b-1C, might be used as a potential vaccine candidate strain.

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