Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Li, Zhen

作者： Li, Zhen;Liu, Xiaoming;Shen, Zhongjian;Fang, Haibo;Zhang, Qingwen;Liu, Xiaoxia;An, Shiheng;Hoover, Kelli;Li, Xiangrui;Ros, Vera I. D.

作者机构：

关键词： 20-hydroxyecdysone; baculovirus; climbing behaviour; Helicoverpa armigera; juvenile hormone; miRNAs

期刊名称：MOLECULAR ECOLOGY （ 影响因子：6.185； 五年影响因子：6.801 ）

ISSN： 0962-1083

年卷期： 2018 年 27 卷 2 期

页码：

收录情况： SCI

摘要： Baculoviruses manipulate host climbing behaviour to ensure that the hosts die at elevated positions on host plants to facilitate virus proliferation and transmission, which is a process referred to as tree-top disease. However, the detailed molecular mechanism underlying tree-top disease has not been elucidated. Using transcriptome analysis, we showed that two hormone signals, juvenile hormone (JH) and 20-hydroxyecdysone (20E), are key components involved in HaSNPV-induced tree-top disease in Helicoverpa armigera larvae. RNAi-mediated knockdown and exogenous hormone treatment assays demonstrated that 20E inhibits virus-induced tree-top disease, while JH mediates tree-top disease behaviour. Knockdown of BrZ2, a downstream signal of JH and 20E, promoted HaSNPV-induced tree-top disease. We also found that two miRNAs target BrZ2 and are involved in the cross-talk regulation between 20E and JH manipulating HaSNPV replication, time to death and HaSNPV-induced tree-top disease.

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