Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6

文献类型： 外文期刊

第一作者： Zheng, Fei

作者： Zheng, Fei;Tu, Tao;Wang, Yuan;Ma, Rui;Su, Xiaoyun;Yao, Bin;Luo, Huiying;Zheng, Fei;Wang, Xiaoyu;Xie, Xiangming

作者机构：

关键词： GH5 cellulase; Saturation mutation; Catalytic activity; Loop region

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN： 1754-6834

年卷期： 2018 年 11 卷

页码：

收录情况： SCI

摘要： Background: Cellulases of glycosyl hydrolase (GH) family 5 share a (beta/alpha)(8) TIM-barrel fold structure with eight beta alpha loops surrounding the catalytic pocket. These loops exposed on the surface play a vital role in protein functions, primarily due to the interactions of some key amino acids with solvent and ligand molecules. It has been reported that motions of these loops facilitate substrate access and product release, and loops 6 and 7 located at the substrate entrance of the binding pocket promote proton transfer reaction at the catalytic site motions. However, the role of these flexible loops in catalysis of GH5 cellulase remains to be explored. Results: In the present study, an acidic, mesophilic GH5 cellulase (with optimal activity at pH 4.0 and 70 degrees C), GtCel5, was identified in Gloeophyllum trabeum CBS 900.73. The specific activities of GtCel5 toward CMC-Na, barley beta-glucan, and lichenan were 1117 +/- 43, 6257 +/- 26 and 5318 +/- 54 U/mg, respectively. Multiple sequence alignment indicates that one amino acid residue at position 233 on the loop 6 shows semi-conservativeness and might contribute to the great catalytic performance. Saturation mutagenesis at position 233 was then conducted to reveal the vital roles of this position in enzyme properties. In comparison to the wild type, variants N233A and N233G showed decreased optimal temperature (-10 degrees C) but increased activities (27 and 70%) and catalytic efficiencies (k(cat)/K-m; 45 and 52%), respectively. The similar roles of position 233 in catalytic performance were also verified in the other two GH5 homologs, TeEgl5A and PoCel5, by reverse mutation. Further molecular dynamics simulations suggested that the substitution of asparagine with alanine or glycine may introduce more hydrogen bonds, increase the flexibility of loop 6, enhance the interactions between enzyme and substrate, and thus improve the substrate affinity and catalytic efficiency. Conclusion: This study proposed a novel cellulase with potentials for industrial application. A specific position was identified to play key roles in cellulase-substrate interactions and enzyme catalysis. It is of great importance for understanding the binding mechanism of GH5 cellulases, and provides an effective strategy to improve the catalytic performance of cellulases.

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