PARTIAL RECOVERY OF MITOCHONDRIAL FUNCTION OF VITRIFIED PORCINE MII STAGE OOCYTES DURING POST-THAW INCUBATION

文献类型： 外文期刊

第一作者： Dai, Jianjun

作者： Dai, Jianjun;Yang, Junhua;Zhang, Shushan;Niu, Yingfang;Chen, Yaning;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Yang, Junhua;Zhang, Shushan;Niu, Yingfang;Chen, Yaning;Wu, Caifeng;Zhang, Defu

作者机构：

关键词： porcine ooctyes; vitrification; mitochondrial function; apoptosis

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2018 年 39 卷 1 期

页码：

收录情况： SCI

摘要： BACKGROUND: The survival of porcine oocytes is still very low after cryopreservation. OBJECTIVE: To investigate whether and when the mitochondrial function of vitrified porcine oocytes could be recovered post-thaw. MATERIALS AND METHODS: Mitochondrial potential Delta Psi m, ROS level, ATP content, apoptotic rate, caspase activity, and parthenogenetics developmental ability of thawed porcine oocytes were measured after culture in vitro for 0, 1, 2 or 4 h. RESULTS: Mitochondrial Delta Psi m after 2 h and 4 h post-thaw culture were 1.19 and 1.26, significantly lower than that of fresh oocytes but much higher than the groups cultured for 0 h and 1 h (P<0.05). Cryopreservation increased the ROS level in oocytes considerably, which decreased only after 2 to 4 h incubation following thaw. ATP content increased gradually over time, and recovered to the level comparable to that of fresh oocytes after 4 h. Pan caspase levels increased after cryopreservation and reached the highest level at 1 h incubation. Thereafter it decreased to a low value, but still higher than fresh oocytes. Oocytes showing an early apoptotic event decreased upon 2 to 4 h incubation. The parthenogenetic cleavage and blastocyst rates were the highest (19.8% and 5.6%) after 2 h incubation. CONCLUSION: The recovery of mitochondrial function could complete after 2 to 4 h post-thaw incubation. Post-thaw incubation for 2 to 4 h reduced apoptotic events and improved parthenogenetic developmental ability of vitrified porcine MII stage oocytes.

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