文献类型： 外文期刊
第一作者： Xiang, Yang
作者： Xiang, Yang;Du, Caifu;Lei, Lei;Xiang, Yang;Jia, Haiyan;Song, Min;Wang, Yun;Ma, Zhengqiang
作者机构：
关键词： Calreticulin; TaCRT1; Arabidopsis; Drought tolerance; ABA sensitivity
期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）
ISSN： 0556-3321
年卷期： 2018 年 50 卷 1 期
页码：
收录情况： SCI
摘要： The TaCRT1 gene is a member of calreticulin (CRT) family in wheat. In our previous study, we showed that transgenic tobacco lines over expressing wheat TaCRT1 showed enhanced tolerance to salt stress. This study aimed to determine whether TaCRT1 over expression would increase drought tolerance in transgenic Arabidopsis. Over expression of TaCRT1 in Arabidopsis plants enhances tolerance to drought stress. However, the transgenic line was found to retard the growth. Moreover, the transgenic line showed decreased water loss but higher sensitivity to exogenous abscisic acid (ABA) compared with the wild type (Col-0). Meanwhile, the transgenic line had the elevated endogenous ABA level. The semi quantitative RT-PCR (sqRT-PCR) analysis showed that transcription levels of ABA-biosynthesizing gene (NCED3) and ABA-responsive gene (ABF3) were higher in the transgenic line than that in the Col-0 under normal condition. The above results implied that the TaCRT1 might be able to used as a potential target to improve the drought tolerance in crops.
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