Generation, safety and immunogenicity of an Actinobacillus pleuropneumoniae quintuple deletion mutant SLWO7 (Delta apxIC Delta apxIIC Delta orf1 Delta cpxAR Delta arcA)

文献类型： 外文期刊

第一作者： Wang, Chunlai

作者： Wang, Chunlai;Liu, Siguo;Yuan, Fangyan;Tian, Yiongxiang;You, Wujin;Bei, Weicheng;Liu, Jinlin;Zhao, Jin

作者机构：

关键词： Actinobacillus pleuropneumoniae; Quintuple deletion mutant; Safety; Protective efficacy

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2018 年 36 卷 14 期

页码：

收录情况： SCI

摘要： We inactivated a virulence determinant, ArcA, in an Actinobacillus pleuropneumoniae quadruple deletion mutant SLW06 (Delta apxIC Delta apxIIC Delta orf1 Delta cpxAR, serovar 1), and a quintuple deletion mutant SLW07 was generated. SLW07 showed decreased adherence to and invasion of host cells, compared to its parent strain SLW06. SLW07 was more sensitive in RAW264.7 macrophage-mediated phagocytosis and clearance. SLW07 was less virulent in mice. An immunization assay indicated that both SLW07 and SLW06 preferentially stimulated T helper cell type 2 response in mice. Live vaccines induced the production of interleukin-6 and tumor necrosis factor-alpha by splenic lymphocytes. Furthermore, the protective immunity of SLW07 was not affected after ArcA mutation. Immunization with SLW07 could provide a complete protection following virulent A. pleuropneumoniae challenge in mice. Our results suggest that SLW07 is a promising live vaccine candidate, which is further attenuated from and shares similar protective efficacy with its quadruple deletion parent SLW06. (C) 2018 Elsevier Ltd. All rights reserved.

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