Hepatopancreas Transcriptome Analysis of Spinibarbus sinensis to Reveal Different Growth-Related Genes

文献类型： 外文期刊

第一作者： Zhou, Bo

作者： Zhou, Bo;Wang, Bin;Yang, Fei;Ling, Leyan;Hou, Mengdan;Liu, Fan;Li, Yu;Luo, Hui;He, Wenping;Ye, Hua

作者机构： Sichuan Acad Agr Sci, Fisheries Inst, Yibin 644000, Peoples R China;Southwest Univ, Coll Fisheries, Key Lab Aquat Sci Chongqing,Minist Educ, Key Lab Freshwater Fish Reprod & Dev, Chongqing 402460, Peoples R China

关键词： Spinibarbus sinensis; hepatopancreas transcriptome; growth; energy metabolism; neuroendocrine regulation

期刊名称：GENES （ 2023影响因子：2.8； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 15 卷 7 期

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收录情况： SCI

摘要： Spinibarbus sinensis, also known as Qingbo, is an important economic fish in China. However, the detailed mechanisms underlying its growth are still unknown. To excavate the genes and signaling pathways related to its growth, we compared the transcriptome profiles of the hepatopancreas tissues of S. sinensis, with two groups of growth rate for evaluation. An average of 66,304,909 and 68,739,585 clean reads were obtained in the fast growth (FG) and slow growth (SG) group, respectively. The differential gene expression analysis results showed that 272 differentially expressed genes (DEGs) were screened between the FG and SG groups, including 101 up-regulated genes and 171 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results showed that GO terms related to metabolic process, organic substance metabolic process, and catalytic activity were enriched, pathway signals related to steroid biosynthesis and protein digestion and absorption were also detected. Meanwhile, the potential key regulatory genes sst2, fndc4, and cckra related to the growth of S. sinensis were screened. Reverse transcript fluorescence quantitative PCR (RT-qPCR) validation of 18 DEGs associated with growth differences showed that the RT-qPCR results were consistent with RNA-seq analysis, and nine genes, stk31, gpr149, angptl1, fstl1, sik1, ror2, nlrc3, pdlim2, and nav2 were significantly expressed in the FG group. bmp1, stc1, gpatch8, sstrt2, s100a1, ktf6, cckar6, sync1, bhlha15, a total of nine genes were significantly expressed in the SG group. This study provides basic information for improving the growth characteristics of S. sinensis and the functional research of candidate genes.

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