Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618(T) and its application in quorum quenching on Pseudomonas aeruginosa PAO1

文献类型： 外文期刊

第一作者： Dong, Weiwei

作者： Dong, Weiwei;Zhao, Shumiao;Dong, Weiwei;Zhao, Shumiao;Dong, Weiwei;Guo, Xiang;Kong, Delong;Zhou, Yiqing;Liu, Xiaoyang;Ruan, Zhiyong;Zhu, Jie;Zhang, Qi

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： N-Acyl homoserine lactones (AHLs) act as the key quorum sensing (QS) signal molecules in gramnegative bacteria, which coordinates gene expression and then activates various processes, including biofilm formation and production of virulence factors in some pathogens. Quorum quenching (QQ), which is the inactivation of the signal molecules by means of enzymatic degradation or modification, inhibits the processes of QS rather than killing the pathogens and is a promising antipathogenic strategy to control the bacterial pathogens. In this study, an AHL lactonase gene (named aiiK) was cloned from Kurthia huakuii LAM0618T and the AHL lactonase AiiK was expressed by Escherichia coli. AiiK exhibits a variable substrate spectrum and efficient degradation of the AHL compounds. The enzyme assays demonstrated that AiiK behaves as an AHL lactonase that can hydrolyze the lactone bond of the AHLs. The total hydrolytic efficiency of AiiK for C-10-HSL is 3.9 s(-1).mM(-1). AiiK can also maintain 20% activity after 12 h incubation at 37 degrees C and demonstrate great resistance to a-chymotrypsin, trypsin, and protease K. Furthermore, AiiK significantly inhibits the biofilm formation and attenuates extracellular proteolytic activity and pyocyanin production of Pseudomonas aeruginosa PAO1, which indicates the potential application of AiiK as a biocontrol agent or an anti-pathogenic drug.

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