Development of an indirect ELISA based on the recombinant Spm2 protein for detection of tropical theileriosis

文献类型： 外文期刊

第一作者： Tian, Zhancheng

作者： Tian, Zhancheng;Du, Xiaoyue;Du, Junzheng;Gao, Shandian;Yu, Ruiming;Hassan, Muhammad Adeel;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Yin, Hong

作者机构：

关键词： Theilerla annulata; ELISA; Spm2; Tropical theileriosis

期刊名称：ACTA TROPICA （ 影响因子：3.112； 五年影响因子：3.002 ）

ISSN： 0001-706X

年卷期： 2018 年 182 卷

页码：

收录情况： SCI

摘要： Tropical theileriosis, caused by Theileria annulata, is distributed worldwide and causes great economic losses in dairy. The reliable diagnostic method is critical for prevention and control of the disease. In this study, a sporozoite and macroschizont gene 2 (spm2) protein from T. annulata was used to develop an indirect ELISA for tropical theileriosis. Specificity test showed that there were no cross-reactions with antibodies raised against other bovine piroplasm species using ELISA or western blotting. The specificity and sensitivity were 98.4% and 98.7%, respectively, with a threshold of 35.5% of the specific mean antibody rate (AbR). Furthermore, a total of 196 field sera samples collected from Xinjiang and Gansu provinces were detected by the spm2 ELISA and LEA. The results obtained with the spm2 ELISA and IFA in this study had the moderate agreement The average positive rates of T. annulata sera samples detected in the present study were close to the prevalence of previous reports in these endemic areas. This indicated that the Spm2 ELISA could be used as a reliable diagnostic tool for serological survey of T. annulata infection in areas where Theileria parve is not present.

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