Identification of two novel foot-and-mouth disease virus cytotoxic T lymphocyte epitopes that can bind six SLA-I proteins

文献类型： 外文期刊

第一作者： Gao, Feng-Shan

作者： Gao, Feng-Shan;Zhai, Xiao-Xin;Jiang, Ping;Gao, Hua;Li, Zi-Bin;Han, Yong;Yang, Jie;Zhang, Zong-hui;Zhang, Qiang;Li, Zi-Bin

作者机构：

关键词： Major histocompatibility complex; Swine leukocyte antigen; Peptide; Cytotoxic T lymphocyte; Epitope; Foot-and-mouth disease virus

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 653 卷

页码：

收录情况： SCI

摘要： Currently available vaccines from inactivated foot-and-mouth disease virus (FMDV) only protect animals by inducing neutralizing antibodies. A vaccine that contains cytotoxic T lymphocytes (CTL) epitopes to induce strong CTL responses might protect animals more effectively. Herein, we used swine leukocyte antigen class I (SLA I) proteins derived from six different strains of domestic pigs to screen and identify shared FMDV CTL epitopes. Four potential FMDV CTL epitopes (Q01, Q02, AS3, and QA4) were confirmed by mass spectrometry. We also determined the antigenicity of these epitopes to elicit cell-mediated immunoresponse by the ELISPOT and CTL assays. Among the four peptides, Q01 and QA4 were found to bind all six SLA-I proteins with strong affinity and elicit significant activity of CTL (P < 0.01). We conclude that Q01 and QA4 peptides are novel shared epitopes that can be recognized by all six SLA-I molecules on representative CTLs.

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