Protective effects of melatonin on the invitro developmental competence of bovine oocytes

文献类型： 外文期刊

第一作者： Pang, Yunwei

作者： Pang, Yunwei;Zhao, Shanjiang;Sun, Yeqing;Jiang, Xiaolong;Hao, Haisheng;Du, Weihua;Zhu, Huabin

作者机构：

关键词： bovine; embryonic development; invitro maturation; melatonin; oocyte

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

ISSN： 1344-3941

年卷期： 2018 年 89 卷 4 期

页码：

收录情况： SCI

摘要： The present study investigated the effects of melatonin on bovine oocyte maturation and subsequent embryonic development invitro. Results showed that the nuclear and cytoplasmic maturation, characterized by first polar body extrusion, normal distribution of cortical granules and mitochondria, as well as increased mitochondrial membrane potential, were significantly improved in 10(-9)mol/L melatonin-treated oocytes. Melatonin supplementation reduced intracellular reactive oxygen species level and enhanced glutathione production. Meanwhile, the presence of melatonin (10(-9)mol/L) during oocyte maturation resulted in a decreased early apoptotic rate in oocytes. After invitro fertilization, oocytes receiving melatonin supplementation exhibited a significantly higher blastocyst formation rate and yielded a markedly lower number of apoptotic cells. Mechanistic explorations showed that addition of 10(-9)mol/L melatonin to invitro maturation media significantly attenuated the transcript level of caspase-3, while the expressions of BCL-2, XIAP, CAT and HSP70 were significantly reinforced in the resultant embryos. Taken together, melatonin ameliorates bovine oocyte maturation potential, and the beneficial effects can affect subsequent embryonic development. The protective role of melatonin may be due to its anti-apoptotic and anti-oxidative activities.

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