Expression profiles of novel genes and microRNAs involved in lipid deposition in chicken's adipocyte

文献类型： 外文期刊

第一作者： Huang, HuaYun

作者： Huang, HuaYun;Zhao, GuiPing;Liu, RanRan;Li, QingHe;Zheng, MaiQing;Wen, Jie;Huang, HuaYun;Li, ShouFeng;Zhao, ZhenHua

作者机构：

关键词： Genes; miRNA; chicken; adipocyte; lipid deposition

期刊名称：ITALIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：2.217； 五年影响因子：2.376 ）

ISSN： 1594-4077

年卷期： 2018 年 17 卷 3 期

页码：

收录情况： SCI

摘要： With the aim of studying the molecular mechanisms underlying lipid deposition in chickens, the expression profiles of eight novel candidate genes and miRNA involved in lipid deposition were examined in the undifferentiated and differentiated adipocyte at 3d, 5d and 10d after inducing differentiation. The level of expression of gga-miR-30 cluster (gga-miR-30a-5p, miR-30c-5p and miR-30e-5p) and miR-17-92 cluster (gga-miR-17-5p, miR-19a-3b and miR-20a-5p), compared to undifferentiated adipocyte, was significantly up-regulated with differentiated adipocyte; expression of miR-103-3p and miR-92-5p was significantly up-regulated with differentiated adipocyte. Seven genes, ACSL1, CYB5A, SEC23A, BRP44L, PLTP, MGLL and GART, compared to undifferentiated adipocyte, were significantly down-regulated with differentiated adipocyte. Correlation analysis of the detected genes and miRNAs indicated BRP44L may be a target of miR-103-3p and it was negatively correlated with miR-103-3p. CYB5A may be a target of miR-30a-5p and it was negatively correlated with miR-30a-5p. SEC23A may be a target of miR-19a-3p and it was negatively correlated with miR-19a-3p. MGLL may be a target of miR-19a-3p and it was negatively correlated with miR-19a-3p. These findings will improve more integrated information of the miRNA and genes in regulating the quantity of lipid deposition in chicken adipocyte.

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