Identification and characterization of a novel B-cell epitope on Aleutian Mink Disease virus capsid protein VP2 using a monoclonal antibody

文献类型： 外文期刊

第一作者： Lu, Taofeng

作者： Lu, Taofeng;Wang, Yuanzhi;Ma, Qin;Yan, Wenzhuo;Zhang, Yuanyuan;Zhao, Lili;Chen, Hongyan;Ge, Junwei

作者机构：

关键词： Aleutian mink disease virus; Monoclonal antibody; Capsid proteins VP2; B-cell epitope; Epitope mapping

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2018 年 248 卷

页码：

收录情况： SCI

摘要： Aleutian mink disease is caused by a highly contagious parvovirus (Aleutian mink disease virus, AMDV). This disease is one of the most commercially important infectious disease worldwide and causes considerable economic losses to mink farmers. The capsid protein VP2 is the major immunogenic antigenic protein of AMDV, and is involved in viral tropism, pathogenicity, and host selection. However, few reports have described the use of VP2-specific monoclonal antibodies (mAbs) in B-cell epitope identification and immunological detection. In this study, we produced a specific mAb, 1G5, against AMDV VP2 protein (amino acids: 200 similar to 588) and characterized its specificity and relative affinity. Six partially overlapping truncated recombinant proteins and seven synthetized peptides were used to identify the epitopes recognized by 1G5. The results indicate that mAb 1G5 can distinguish AMDV, MEV and CPV2 with high affinity (K-a = 5.37 x 10(9)), and the minimal linear epitope is located in amino acid residues (459)EEEGWPAASGTHFED(473). Sequence alignments demonstrated that the linear epitope was completely conserved among most Amdoparvoviruses except the bat parvovirus, where three substitutions (W-463 E-463 (466)A-(466)G and F-471-Y-471) were noted. Our results reveal that the identified epitope might be a common B-cell epitope of AMDV antibodies, and the 1G5 mAb can be used to identify the cleavage of the capsid proteins during AMDV infection. This is also the first report of a B-cell epitope on AMDV capsid protein VP2 (VP2: 459-473) using a mAb. These findings have potential applications in the development of new diagnostic tools for AMDV.

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