Identification of 'Xinlimei' radish candidate genes associated with anthocyanin biosynthesis based on a transcriptome analysis
文献类型： 外文期刊
第一作者： Sun, Yuyan
作者： Sun, Yuyan;Wang, Jinglei;Qiu, Yang;Liu, Tongjin;Song, Jiangping;Li, Xixiang;Sun, Yuyan
作者机构：
关键词： Raphanus sativus L.; Taproot; Anthocyanin biosynthesis; Transcriptome sequencing; qRT-PCR
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2018 年 657 卷
页码：
收录情况： SCI
摘要： Radish is an economically important vegetable crop belonging to the family Brassicaceae. The high anthocyanin content of the 'Xinlimer radish roots has been associated with diverse health benefits. However, there is a lack of transcript-level information regarding anthocyanin biosynthesis. In the present study, the 'Xinlimei' radish root transcriptome was analyzed by RNA sequencing at five developmental stages. A total of 222,384,034 clean reads were obtained and 32,253 unigenes were annotated. Expression profiles revealed 10,890 differentially expressed genes (DEGs) among the five analyzed libraries. The DEGs were predominantly involved in KEGG pathways related to the biosynthesis of phenylpropanoids, flavonoids, flavone, and flavonol. The transcriptome data revealed 44 structural and 182 transcription factor genes (TFs) associated with the anthocyanin biosynthetic pathway. Ten structural genes (i.e., 4CL3, CHSB2, CHS1, CHS3, F3H1, F3'H, DFR, DFR1, ANS, and UFGT) and two MYB genes, which were highly and differentially expressed during root development, may be critical for anthocyanin biosynthesis. Additionally, the co-expression of TFs and structural genes was analyzed. Three structural genes (i.e., DFR, ANS, and UFGT) were validated by molecular cloning. The qRT-PCR results indicated that the expression profiles of DEGs were generally consistent with the high-throughput sequencing results. These findings helped identify candidate genes involved in anthocyanin biosynthesis and may be useful for clarifying the molecular mechanism underlying the accumulation of anthocyanins in radish roots.
分类号：
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis
作者：Ai, Jiaqi;Hu, Na;Ai, Jiaqi;Wang, Wuhong;Hu, Tianhua;Hu, Haijiao;Yan, Yaqin;Wang, Jinglei;Wang, Yunzhu;Hu, Na;Pang, Hongtao;Bao, Chonglai;Wei, Qingzhen
关键词：eggplant; MYB gene family; genome-wide identification; anthocyanin; RNA-seq
-
Comparative Analysis of Prediction Models for Trawling Grounds of the Argentine Shortfin Squid Illex argentinus in the Southwest Atlantic High Seas Based on Vessel Position and Fishing Log Data
作者：Xiang, Delong;Sun, Yuyan;Zhu, Hanji;Wang, Jianhua;Huang, Sisi;Zhang, Shengmao;Zhang, Famou;Zhang, Heng;Xiang, Delong;Sun, Yuyan;Huang, Sisi;Zhang, Shengmao;Zhang, Famou;Zhang, Heng;Xiang, Delong;Sun, Yuyan;Huang, Sisi;Zhang, Shengmao;Zhang, Famou;Zhang, Heng;Zhu, Hanji;Wang, Jianhua
关键词：Southwest Atlantic;
Illex argentinus ; AIS; deep learning; fishing ground prediction -
Construction of SNP Fingerprinting and Genetic Diversity Analysis of Eggplant Based on KASP Technology
作者：Wang, Wuhong;Pang, Hongtao;Hu, Na;Hu, Haijiao;Hu, Tianhua;Yan, Yaqin;Wang, Jinglei;Ai, Jiaqi;Bao, Chonglai;Wei, Qingzhen
关键词：eggplant; KASP; DNA fingerprinting; genetic diversity; population structure
-
Integrated Analysis of the Transcriptome and Metabolome Reveals the Network Regulating Fruit Taste in Sponge Gourd (Luffa cylindrica)
作者：Chai, Yaqian;Qiu, Wenjing;Gao, Luyao;Dong, Wenqi;Zhang, Peng;Zhou, Shengjun;Wang, Xin;Zhu, Yuqiang;Sun, Yuyan;Chai, Yaqian;Li, Zhikun
关键词：sponge gourd; taste; transcriptome; metabolome; mechanism
-
Simulation and Identification of the Habitat of Antarctic Krill Based on Vessel Position Data and Integrated Species Distribution Model: A Case Study of Pumping-Suction Beam Trawl Fishing Vessels
作者：Zhang, Heng;Sun, Yuyan;Zhu, Hanji;Xiang, Delong;Wang, Jianhua;Zhang, Famou;Huang, Sisi;Li, Yang;Zhang, Heng;Xiang, Delong;Huang, Sisi;Zhang, Heng;Zhu, Hanji;Wang, Jianhua;Li, Yang;Sun, Yuyan;Xiang, Delong;Zhang, Famou;Huang, Sisi
关键词：Antarctic krill; vessel position data; environmental data; species distribution model; habitat research
-
Comparative analysis of modeling methods and prediction accuracy for Japanese sardine habitat under three climate scenarios with differing greenhouse emission pathways
作者：Sun, Yuyan;Xiang, Delong;Wang, Jianhua;Jiang, Keji;Zhu, Hanji;Huang, SiSi;Zhang, Famou;Li, Yang;Zhang, Heng;Sun, Yuyan;Xiang, Delong;Jiang, Keji;Huang, SiSi;Zhang, Heng;Sun, Yuyan;Xiang, Delong;Jiang, Keji;Huang, SiSi;Zhang, Famou;Zhang, Heng;Wang, Jianhua;Zhu, Hanji;Li, Yang
关键词：Japanese sardine; Species distribution models (SDMs); Climate change; Spatial resolution; Species presence data points
-
Homozygous editing of multiple genes for accelerated generation of xenotransplantation pigs
作者：Duan, Xiaoyue;Guo, Liang;Hou, Naipeng;Li, Pan;Gao, Fei;Du, Xuguang;Song, Jiangping;Wu, Sen;Duan, Xiaoyue;Chen, Chaolei;Du, Chang;Guo, Liang;Liu, Jun;Li, Pan;Gao, Fei;Du, Xuguang;Wu, Sen;Qi, Xiaolan;Song, Jiangping;Wu, Sen
关键词：
