Molecular cloning and functional characterization of myogenin promoter in Min pig

文献类型： 外文期刊

第一作者： Zhang, X.

作者： Zhang, X.;Liu, D.;Zhang, D.;Wang, L.;Liu, D.

作者机构：

关键词： C2C12 cell; Min pig; myogenin gene; promoter

期刊名称：ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE （ 影响因子：0.684； ）

ISSN： 0906-4702

年卷期： 2017 年 67 卷 1-2 期

页码：

收录情况： SCI

摘要： Myogenin (MyoG) plays an important role in muscle differentiation, which can initiate the formation of the fusion of muscle cells and myofibroblasts. To investigate the transcriptional regulatory mechanism of MyoG gene in Min pig, the promoter fragment spanning from -2085bp to +118bp was amplified with PCR, and the potential transcriptional elements were predicted via bioinformatics analysis. Subsequently, nine promoter segments with different base pair sizes were obtained and ligated into PGL3-Basic vectors, respectively. Lastly, the functional activity of each of the aforementioned promoters was tested separately in transiently transfected C2C12 cells via a dual-luciferase assay system. The results indicated that the sequence -199bp to -31bp may have the basic activity of the promoter. Further analysis demonstrated that the sequence -137bp to -101bp was important for the basic transcriptional activity. Bioinformatics analysis showed that CdxA and MZF1 transcription factors may play an important role in the regulation of MyoG expression.

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