Myocyte enhancer factor 2A promotes proliferation and its inhibition attenuates myogenic differentiation via myozenin 2 in bovine skeletal muscle myoblast

文献类型： 外文期刊

第一作者： Wang, Ya-Ning

作者： Wang, Ya-Ning;Yang, Wu-Cai;Li, Pei-Wei;Wang, Hong-Bao;Zan, Lin-Sen;Wang, Ya-Ning;Yang, Wu-Cai;Li, Pei-Wei;Wang, Hong-Bao;Zan, Lin-Sen;Zhang, Ying-Ying;Zan, Lin-Sen

作者机构： Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China;Natl Beef Cattle Improvement Ctr China, Yangling, Shaanxi, Peoples R China;Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai, Peoples R China;Natl & Prov Joint Engn Res Ctr Modern Cattle Biot, Yangling, Shaanxi, Peoples R China

期刊名称：PLOS ONE （ 2020影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Myocyte enhancer factor 2A (MEF2A) is widely distributed in various tissues or organs and plays crucial roles in multiple biological processes. To examine the potential effects of MEF2A on skeletal muscle myoblast, the functional role of MFE2A in myoblast proliferation and differentiation was investigated. In this study, we found that the mRNA expression level of Mef2a was dramatically increased during the myogenesis of bovine skeletal muscle primary myoblast. Overexpression of MEF2A significantly promoted myoblast proliferation, while knockdown of MEF2A inhibited the proliferation and differentiation of myoblast. RT-PCR and western blot analysis revealed that this positive effect of MEF2A on the proliferation of myoblast was carried out by triggering cell cycle progression by activating CDK2 protein expression. Besides, MEF2A was found to be an important transcription factor that bound to the myozenin 2 (MyoZ2) proximal promoter and performed upstream of MyoZ2 during myoblast differentiation. This study provides the first experimental evidence that MEF2A is a positive regulator in skeletal muscle myoblast proliferation and suggests that MEF2A regulates myoblast differentiation via regulating MyoZ2.

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