Nitric oxide regulates chlorophyllide biosynthesis and singlet oxygen generation differently between Arabidopsis and barley

文献类型： 外文期刊

第一作者： Zhang, Zhong-Wei

作者： Zhang, Zhong-Wei;Li, Meng-Xia;Feng, Ling-Yang;Fu, Yu-Fan;Zheng, Xiao-Jian;Peng, Hong-Qian;Yuan, Shu;Huang, Bo;Chen, Yang-Er;Yuan, Ming;Wu, Fan;Yang, Hai-Ning;Wu, Lin-Tao

作者机构：

关键词： Arabidopsis; Barley; NADPH:protoclilorophyllide oxidoreductase; Singlet oxygen; S-nitrosylation

期刊名称：NITRIC OXIDE-BIOLOGY AND CHEMISTRY （ 影响因子：4.427； 五年影响因子：4.505 ）

ISSN： 1089-8603

年卷期： 2018 年 76 卷

页码：

收录情况： SCI

摘要： Nitric oxide (NO) has a general inhibitory effects on chlorophyll biosynthesis, especially to the step of 5-aminolevulinic acid (ALA) biosynthesis and protochlorophyllide (Pchlide) to chlorophyllide (Chlide) conversion (responsible by the NADPH:Pchlide oxidoreductase POR). Previous study suggested that barley large POR aggregates may be generated by dithiol oxidation of cysteines of two POR monomers, which can be disconnected by some reducing agents. POR aggregate assembly may be correlated with seedling greening in barley, but not in Arabidopsis. Thus, NO may affect POR activity and seedling greening differently between Arabidopsis and barley. We proved this assumption by non-denaturing gel-analysis and reactive oxygen species (ROS) monitoring during the greening. NO treatments cause S-nitrosylation to POR cysteine residues and disassembly of POR aggregates. This modification reduces POR activity and induces Pchlide accumulation and singlet oxygen generation upon dark-to-high-light shift (and therefore inducing photobleaching lesions) in barley leaf apex, but not in Arabidopsis seedlings. ROS staining and ROS-related-gene expression detection confirmed that superoxide anion and singlet oxygen accumulated in barley etiolated seedlings after the NO treatments, when exposed to a fluctuating light. The data suggest that POR aggregate assembly may be correlated with barley chlorophyll biosynthesis and redox homeostasis during greening. Cysteine S-nitrosylation may be one of the key reasons for the NO-induced inhibition to chlorophyll biosynthetic enzymes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序