Rapid specific and visible detection of porcine circovirus type 3 using loop-mediated isothermal amplification (LAMP)
文献类型： 外文期刊
第一作者： Zheng, S.
作者： Zheng, S.;Wu, X.;Shi, J.;Peng, Z.;Xin, C.;Xu, S.;Han, H.;Yu, J.;Sun, W.;Cong, X.;Li, J.;Wang, J.;Zheng, S.;Xin, C.;Gao, M.;Liu, Y.;Wang, S.
作者机构：
关键词： detection; loop-mediated isothermal amplification (LAMP); PCV3
期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）
ISSN： 1865-1674
年卷期： 2018 年 65 卷 3 期
页码：
收录情况： SCI
摘要： In this study, a rapid and specific assay for the detection of porcine circovirus type 3 (PCV3) was established using loop-mediated isothermal amplification (LAMP). Four primers were specifically designed to amplify PCV3. The LAMP assay was effectively optimized to amplify PCV3 by water bath at 60 degrees C for 60min. The detection limit was approximately 1x10(1) copy in this LAMP assay. Compared to porcine circovirus type 2 (PCV2), both gE and gD genes of pseudorabies virus (PRV) and porcine parvovirus (PPV), the LAMP assay showed a high specific detection of PCV3. A visible detection method was developed using SYBR Green I to recognize the results rapidly. Based on the detection of 20 clinical tissue samples, the LAMP assay was more practical and convenient than classical PCR due to its simplicity, high sensitivity, rapidity, specificity, visibility and cost efficiency.
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