Regulation of rho-coumaric acid tolerance in Clostridium beijerinckii by disturbing the intracellular electron transport chain

文献类型： 外文期刊

第一作者： Liu, Jun

作者： Liu, Jun;Chai, Xueying;Luo, Yunchuan;Guo, Ting;Liu, Jun;Ying, Hanjie;Liu, Zuojun

作者机构：

关键词： C. beijerinckii; rho-coumaric acid; Tolerance; Adaptation; NADH

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2018 年 68 卷

页码：

收录情况： SCI

摘要： rho-coumaric acid with strong antibacterial activity is produced during the pretreatment and hydrolysis of lignocellulosic biomass to monomeric sugars. The cell growth and metabolism of C. beijerinckii NCIMB 8052 is significantly inhibited by rho-coumaric acid. In this study, adaptation of C. beijerinckii NUMB 8052 to rho-coumaric acid was remarkably enhanced after disruption of the intracellular electron transport chain, and the antibacterial activity assay showed a 2-fold increase in cell viability in the presence of 0.5 g/L rho-coumaric acid 24 h after inoculation. Additionally, electricity generation in the wild-type and recombinant strains was measured using microbial fuel cell devices, and the intracellular levels of co-factor NAD(P)H were determined by the enzyme cycling method to show disturbed intracellular electron transfer after disruption of gene Cbei_2996. Furthermore, the metabolism of rho-coumaric acid by C. beijerinckii was analyzed. These data indicated that gene Cbei_2996 plays a significant role in regulating rho-coumaric add tolerance in C. beijerinckii; the metabolism of pcoumaric acid mainly coupled with co-factor NADH is catalyzed by reductase. The strategy used in this study provides a potential approach for producing dominant microorganisms with high inhibitor tolerance for butanol production using renewable lignocellulosic materials.

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