Identification and mRNA Expression of Antioxidant Enzyme Genes in the Mud Crab (Scylla paramamosain) in Response to Acute Ammonia and Nitrite Exposure

文献类型： 外文期刊

第一作者： Cheng, Chang-Hong

作者： Cheng, Chang-Hong;Ma, Hong-Ling;Feng, Juan;Su, Yu-Lu;Deng, Yi-Qing;Peng, Jun-Hui;Guo, Zhi-Xun;Guo, Zhi-Xun

作者机构：

关键词： Scylla paramamosain; antioxidant enzyme genes; thioredoxin reductase; ambient stressors

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.287； 五年影响因子：0.593 ）

ISSN： 0792-156X

年卷期： 2018 年 70 卷

页码：

收录情况： SCI

摘要： Thioredoxin reductase (TrxR) is a conserved protein that is involved in protecting organisms against various oxidative stresses. In this study, a thioredoxin reductase gene was cloned from the mud crab Scylla paramamosain (SpTrxR). The full-length cDNA of SpTrxR is comprised of 2724 bp with a 1791 bp open reading frame that encodes a putative protein of 596 amino acids. The deduced amino acid sequence of SpTrxR contains the typical TrxR domain. Quantitative real-time PCR analysis revealed that the SpTrxR mRNA was distributed abundantly in mud crabs, while strong expression was observed mainly in the gills. The expression of antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) was measured using quantitative real-time PCR after acute ammonia and nitrite exposure. The results show that antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) were modulated by acute ammonia and nitrite exposure. These results suggest that antioxidant enzyme genes play an important role in protecting organisms against oxidative stress.

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