Molecular Cloning and Expression of Major Histocompatibility Complex Class Ia Gene of Swamp Eel (Monopterus albus)

文献类型： 外文期刊

第一作者： Pan, Ting-shuang

作者： Pan, Ting-shuang;Jiang, He;Duan, Guo-qing;Hu, Yu-ting;Ling, Jun;Zhou, Hua-xing

作者机构：

关键词： swamp eel; MHC Ia; genomic structure; Aeromonas hydrophila; infection

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.287； 五年影响因子：0.593 ）

ISSN： 0792-156X

年卷期： 2018 年 70 卷

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) plays an important role in the immune response of vertebrates. In this study, we isolated MHC class Ia gene from swamp eel (Monopterus albus) by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). In order to study the function of MHC Ia in swamp eel, tissue distribution and immune response of the MHC Ia after bacterial challenge were analyzed. All the characteristic features of MHC Ia chain structure were identified by the deduced amino acid sequence, including the leader peptide, a1, a2, and a3 domains, connecting peptide, transmembrane and cytoplasmic regions, conserved cysteines, and N-glycosylation site. The deduced amino acid sequence of the MHC Ia shares 27.3% to 69.6% similarity with those of other vertebrates. Quantitative real-time PCR (qRT-PCR) demonstrated that MHC Ia gene was ubiquitously expressed in ten tissues, comparatively higher in immune related tissues, including the liver and intestine. Challenge of M. albus with the extracellular pathogen, Aeromonas hydrophila, resulted in a significant increase in the expression of MHC Ia within 72 h after infection and these high levels were maintained for 72 h in kidneys, spleen, and skin, whereas in the intestine and liver, it was found first in the form of up-regulated and then down-regulated gene. These results demonstrated that MHC Ia gene played an important role in response to bacterial infection in M. albus.

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