Identification of sea cucumber species in processed food products by PCR-RFLP method

文献类型： 外文期刊

第一作者： Zeng, Ling

作者： Zeng, Ling;Chen, Ziming;Wen, Jing;Sun, Yulin;Chen, Daohai;Zhao, Juan;Xu, Lele;Li, Yongqin;Fan, Sigang;Xu, Youhou

作者机构：

关键词： Sea cucumbers; Species identification; 16S rRNA gene; PCR-RFLP; FINS

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 90 卷

页码：

收录情况： SCI

摘要： A PCR-RFLP method has been developed for the identification of 16 commercial sea cucumber species in raw and processed food products. To implement the method, PCR amplification with the 16Sar/16Sbr primers, targeted to the amplification of a ca. 570 bp region of the 16S rRNA mitochondrial gene in sea cucumbers, was coupled to restriction analysis with Dde I, Hae Ill and Sty I. We also report the FINS method based on 16S rRNA mitochondrial sequences. Both proposed methodologies were independently applied to authenticate the species of 19 commercial products, showing that 9 products were incorrectly labeled (48%). Therefore, the 16S rRNA mitochondrial gene really provide convenient, useful and academic molecular marker to study questions related to correct labeling of commercial products, traceability, and the control of sea cucumber fisheries. (C) 2018 Elsevier Ltd. All rights reserved.

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