qNCLB7.02, a novel QTL for resistance to northern corn leaf blight in maize

文献类型： 外文期刊

第一作者： Wang, Jianjun

作者： Wang, Jianjun;Xu, Zhennan;Yang, Jing;Lu, Xiaohuan;Zhou, Zhiqiang;Zhang, Chaoshu;Zheng, Lei;Tian, Ran;Hao, Zhuanfang;Yong, Hongjun;Li, Mingshun;Zhang, Degui;Li, Xinhai;Weng, Jianfeng;Xu, Zhennan;Zhang, Chaoshu

作者机构：

关键词： Maize; Northern corn leaf blight; QTL (quantitative trait loci); Recombinant inbred line; Chromosome segment substitution line

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2018 年 38 卷 5 期

页码：

收录情况： SCI

摘要： Northern corn leaf blight (NCLB), which is caused by the hemibiotrophic fungal pathogen Setosphaeria turcica, is a devastating foliar disease that results in considerable maize yield losses. In the present study, quantitative trait locus (QTL) analysis was conducted across two environments using an ultra-high density bin map constructed using recombinant inbred lines (RILs) derived from a cross between Ye478 and Qi319. A total of 11 QTLs, located on chromosomes 1,4, 5,6, 7, 8, 9, and 10, were detected that confer resistance to physiological race 0 of NCLB. Each QTL could explain 3.53-15.29% of the total phenotypic variation in disease resistance after artificial inoculation in two environments. Among these QTL, qNCLB7.02, which is located on chromosome 7, had the largest effect, accounting for 10.11 and 15.29% of the phenotypic variation in resistance in two field trials and BLUP. The common confidence interval (CI) for qNCLB7.02 was 1.4 Mb, according to the B73 RefGen_v3 sequence. The resistance effect of qNCLB7.02 was validated in 2016 by using chromosome segment substitution lines (CSSLs) derived from Qi319 as the donor in the genetic background of Ye478. The type 6 CSSL, which harbors introgressed qNCLB7.02, was found to be significantly associated with resistance to NCLB by linked marker bnlg 1808 and exhibited greater resistance than the other CSSLs that did not carry this QTL (P = 0.0008). The combination of linkage mapping in RILs and validation in CSSLs is a powerful approach for the dissection of QTL for disease resistance in maize.

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