Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum

文献类型： 外文期刊

第一作者： Yuling Liu

作者： Yuling Liu;Liu, Fang;Peng, Renhai;Baohong Zhang;Xinpeng Wen;Shulin Zhang;Yangyang Wei;Quanwei Lu;Zhen Liu;Kunbo Wang;Fang Liu;Renhai Peng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 5 期

页码：

收录情况： SCI

摘要： A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD(4)) was constructed. Intact nuclei from G. mustelinum (AD(4)) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 x genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.

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