An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection

文献类型： 外文期刊

第一作者： Zhang Lijuan

作者： Zhang Lijuan;Li Ningqiu;Lin Qiang;Liu Lihui;Liang Hongru;Huang Zhibin;Fu Xiaozhe;Zhang Lijuan;Zhang Lijuan;Li Ningqiu;Lin Qiang;Liu Lihui;Liang Hongru;Huang Zhibin;Fu Xiaozhe

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关键词： Rhabdovirus; MSRV-SS; Chinese perch; Live vaccine; Relative percent survival

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 77 卷

页码：

收录情况： SCI

摘要： In order to develop live vaccine against Siniperca chuatsi rhabdovirus (SCRV) disease, an avirulent virus strain, designed as Micropterus salmoides rhabdovirus Sanshui (MSRV-SS), was selected from six fish rhabdovirus isolates (SCRV-QY, SCRV-SS, SCRV-GM, CMRV-FS, OMBRV-JM, MSRV-SS) by fish challenge assay. When Chinese perch (Siniperca chuatsi) were intraperitoneally injected live virus strain MSRV-SS, they were completely protected from virulent SCRV-GM challenge with a relative percent survival (RPS) of 100% on 18th day post vaccination. Then, the wild type MSRV-SS was purified by plaque clone assays, and the biological characteristics of the clonal strain designed as MSRV-SS-7 were investigated. The MSRV-SS-7 was avirulent to Chinese perch and its growth characteristic was similar to the MSRV-SS. The immune protection effects of clonal MSRV-SS-7 against virulent SCRV-GM were evaluated by intraperitoneal injection (IP) vaccination and immersion (IM) vaccination, their RPSs were all 100%. Altogether, these results indicate that MSRV-SS-7 is a potential live vaccine candidate against SCRV disease.

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