Simultaneous detection of Theileria annulata and Theileria orientalis infections using recombinase polymerase amplification

文献类型： 外文期刊

第一作者： Hassan, Muhammad Adeel

作者： Hassan, Muhammad Adeel;Liu, Junlong;Rashid, Muhammad;Guan, Guiquan;Yin, Hong;Luo, Jianxun;Sajid, Muhammad Sohail;Sajid, Muhammad Sohail;Mahmood, Altaf;Abbas, Qamar;Yin, Hong

作者机构：

关键词： Recombinase polymerase amplification; Theileria annulata; Theileria orientalis; PCR; Pakistan

期刊名称：TICKS AND TICK-BORNE DISEASES （ 影响因子：3.744； 五年影响因子：3.693 ）

ISSN： 1877-959X

年卷期： 2018 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Theileriosis is a disease of domesticated animals in tropical and subtropical countries and causes significant reductions in livestock productivity. The arid region of Punjab in Pakistan is notorious for the presence of the vector tick (Acari: Ixodidae) and tick-borne diseases, such as theileriosis and babesiosis. The distribution of Theileria annulata and T. orientalis in the Chakwal district of Punjab was determined by developing a multiplex recombinase polymerase amplification (RPA) assay as a scientific basis for formulating control strategies for bovine theileriosis. Specificity was evaluated using DNA from related piroplasm species, while analytical sensitivity was calculated using a long fragment of the enolase gene. A total of 188 blood samples were collected on FTA cards (Whatman (R)) from tick-infested asymptomatic breeds of cattle (Bos indicus, Bos taurus, and Bos indicus x Bos taurus) in the study district. Finally, infections with of T. annulata and T. orientalis were detected using the multiplex RPA and compared with the conventional multiplex polymerase chain reaction (PCR). The multiplex RPA specifically amplified 282-bp and 229-bp fragments of the enolase gene from T. annulata and T. orientalis and had no cross-reaction with other piroplasm species. It was determined that 45 (23.9%) and 5 (2.6%) out of 188 blood samples were positive for T. annulata and T. orientalis, respectively, when examined using RPA. Multiplex PCR detection indicated that 32 (17.0%) and 3 (1.6%) blood samples were positive for T. annulata and T. orientalis, respectively. In the present study, a specific RPA method was developed for simultaneous differentiation and detection of T. annulata and T. orientalis infections and used for the first time for the detection of the two bovine Theileria infections.

分类号：

  • 相关文献
作者其他论文 更多>>
  • RGB imaging and computer vision-based approaches for identifying spike number loci for wheat

    作者：Li, Lei;Wang, Duoxia;Wan, Guoliang;Rasheed, Awais;Xia, Xianchun;Zhang, Yong;He, Zhonghu;Liu, Jindong;Xiao, Yonggui;Li, Lei;Zhang, Yong;Liu, Jindong;Li, Lei;He, Yong;Hassan, Muhammad Adeel;Beegum, Sahila;Hassan, Muhammad Adeel;Beegum, Sahila;Rasheed, Awais;Rasheed, Awais;He, Zhonghu

    关键词：Common wheat; Imaging; KASP markers; Quantitative genetic analysis; Spike number

  • Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics

    作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong

    关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health

  • A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China

    作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong

    关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity

  • High-throughput phenotyping discovers new stable loci controlling senescence rate in bread wheat

    作者：Li, Lei;Liu, Jindong;Wang, Duoxia;Fei, Shuaipeng;Zeng, Jianqi;Rasheed, Awais;Xia, Xianchun;He, Zhonghu;Zhang, Yong;Xiao, Yonggui;Li, Lei;Zhang, Yong;Li, Lei;Wang, Keyi;He, Yong;Hassan, Muhammad Adeel;Hassan, Muhammad Adeel;Rasheed, Awais;He, Zhonghu;Rasheed, Awais

    关键词：Aerial digital imaging; Active accumulated temperature; Chlorophyll; QTL; Senescence rate; Common wheat

  • Expanding the molecular understanding of Theileria infections in cattle and yaks from Gansu and Qinghai, China

    作者：Chai, Yijun;Che, Jin;Zhao, Shuaiyang;Wang, Jinming;Guan, Guiquan;Yin, Hong;Che, Jin;Yin, Hong

    关键词：Molecular prevalence; Theileriosis; Piroplasm; Bovine

  • Lacticaseibacillus rhamnosus G7 alleviates DSS-induced ulcerative colitis by regulating the intestinal microbiota

    作者：Lao, Jianlong;Chen, Man;Yan, Shuping;Gong, Han;Wen, Zhaohai;Yong, Yanhong;Jia, Dan;Lv, Shuting;Zou, Wenli;Li, Junmei;Tan, Huiming;Liu, Zengyuan;Guo, Fucheng;Ju, Xianghong;Li, Youquan;Lao, Jianlong;Ju, Xianghong;Li, Youquan;Jia, Dan;Yin, Hong;Kong, Xiangying

    关键词：Probiotics; Inflammatory; Lacticaseibacillus rhamnosus G7; Gut microbiota

  • First determination of diagnostic doses of the pesticide-sensitive strain of Haemaphysalis longicornis in China

    作者：Diao, Peiwen;Luo, Jin;Ren, Qiaoyun;Wang, Jinming;Cao, Runlai;Guo, Junhui;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Liu, Guangliang;Liu, Guangyuan;Ren, Qiaoyun;Zhang, Yan;Luo, Jianxun;Yin, Hong

    关键词：ticks; pesticide-sensitive strain; Haemaphysalis longicornis; discriminating concentration

微信小程序