Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro

文献类型： 外文期刊

第一作者： Li, Shi-fang

作者： Li, Shi-fang;Shao, Jun-jun;Zhao, Fu-rong;Gong, Mei-jiao;Xie, Yin-li;Chang, Hui-yun;Zhang, Yong-guang;Li, Shi-fang;Shao, Jun-jun;Zhao, Fu-rong;Xie, Yin-li;Chang, Hui-yun;Zhang, Yong-guang;Gong, Mei-jiao

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关键词： Type I interferon; Porcine interferon-delta; Antiviral activity; FMDV

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：4.932； 五年影响因子：4.624 ）

ISSN： 1567-5769

年卷期： 2018 年 59 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) is one of the most devastating diseases affecting livestock. Since vaccines fail to provide protection until seven days post-vaccination, the application of anti-viral molecules is imperative for suppressing the spread of FMDV prior to development of an adaptive immune response. Interferons (IFNs) are effective for the host to fight FMDV infections; however, a novel type I IFNs, interferon delta (IFN-delta), has not been investigated for their antiviral effects against this virus. Thus, this study investigated FMDV infection, upon pre- and post-treatment with PoIFN-delta 8. Real-time quantitative PCR was used to quantify the expression levels of IFN-stimulated genes (ISG5), including ISG15, OAS1, PKR, and Mx1. Results showed the PolFN-delta 8 lacking its signal sequence was efficiently expressed in Escherichia coh., and the purified recombinant PoIFN-delta 8 exerted a significantly protective effect against two different serotypes of FMDV in IBRS-2 cells. In addition, PoIFN-delta 8 induced the expression of IFN-stimulated genes. These findings highlight the significance of PoIFN-delta might serve as an antiviral agent for the prevention of FMDV in pigs and will stimulate the study of exploiting the potential biological functions of IFN-delta in the future.

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