An alkaline and surfactant-tolerant lipase from Trichoderma lentiforme ACCC30425 with high application potential in the detergent industry
文献类型： 外文期刊
第一作者： Wang, Yuzhou
作者： Wang, Yuzhou;Li, Shigui;Gong, Mingbo;Gu, Jingang;Ma, Rui;Yao, Bin;Bai, Yingguo
作者机构：
关键词： Trichoderma lentiforme; Alkaline lipase; Heterologous expression; Detergent
期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）
ISSN： 2191-0855
年卷期： 2018 年 8 卷
页码：
收录情况： SCI
摘要： Alkaline lipases with adaptability to low temperatures and strong surfactant tolerance are favorable for application in the detergent industry. In the present study, a lipase-encoding gene, TllipA, was cloned from Trichoderma lentiforme ACCC30425 and expressed in Pichia pastoris GS115. The purified recombinant TlLipA was found to have optimal activities at 50 degrees C and pH 9.5 and retain stable over the pH range of 6.0-10.0 and 40 degrees C and below. When using esters of different lengths as substrates, TlLipA showed preference for the medium length p-nitrophenyl octanoate. In comparison to commercial lipases, TlLipA demonstrated higher tolerance to various surfactants (SDS, Tween 20, and Triton X100) and retained more activities after incubation with Triton X100 for up to 24 h. These favorable characteristics make TlLipA prospective as an additive in the detergent industry.
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