Fine mapping of a major QTL controlling early flowering in tomato using QTL-seq

文献类型： 外文期刊

第一作者： Ruangrak, Eaknarin

作者： Ruangrak, Eaknarin;Ruangrak, Eaknarin;Ruangrak, Eaknarin;Su, Xiaomei;Huang, Zejun;Wang, Xiaoxuan;Guo, Yanmei;Du, Yongchen;Gao, Jianchang

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关键词： tomato; early flowering loci; QTL-seq

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2018 年 98 卷 3 期

页码：

收录情况： SCI

摘要： Early flowering is one of the major earliness traits in tomato and is also an important agronomical trait in crop plants; thus, this trait is important for plant breeding and crop improvement. With the innovation of rapid and cost-effective technologies, quantitative trait locus (QTL)-seq has become the preferred method of performing QTL identification. In the present study, we identified a candidate QTL of an early flowering trait in tomato (Solanum lycopersicum) using QTL-seq. Two DNA pools of the extreme phenotype of the F-2 progeny from crosses between the 'Bone MM' cultivar (early flowering, P-1) and '071-440' cultivar (late flowering, P-2) were bulked for sequencing and an alignment analysis. We observed 220 single nucleotide polymorphism markers, seven candidate QTLs, and genes that may be associated with early flowering located between 1.6 and 71.8 Mb on chromosome 1. Using traditional QTL analysis, the location of one QTL was confirmed in the physical region between 23.5 and 25.3 Mb, which corresponded to the region identified using QTL-seq, and was referred to as EF1 (Solyc01g017060). A real-time quantitative reverse transcription polymerase chain reaction analysis showed that EF1 was the most highly expressed among the candidate genes and significantly expressed in early flowering parents and furthermore, we found that EF1, which had a similar sequence to the Ycf2 gene, may relate to the early flowering phenotype.

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