Haploid plant regeneration from hardy kiwifruit (Actinidia arguta Planch.) anther culture

文献类型： 外文期刊

第一作者： Wang, Guang-Fu

作者： Wang, Guang-Fu;Qin, Hong-Yan;Sun, Dan;Fan, Shu-Tian;Yang, Yi-Ming;Wang, Zhen-Xing;Xu, Pei-Llei;Zhao, Ying;Liu, Ying-Xue;Ai, Jun

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关键词： Actinidia arguta; Anther culture; Ploidy identification

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2018 年 134 卷 1 期

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收录情况： SCI

摘要： Haploids are important for studying plant breeding and genome sequencing. Regeneration from in vitro anther culture under aseptic conditions has achieved success in many higher plants. This study focused on anther culture of hardy kiwifruit (Actinidia arguta Planch.) to obtain haploid or double haploid plants that originated from microspores. Using the anthers of hardy kiwifruit as the test materials, we report the relationship between the microspore development stage and the bud morphological characteristics, some factors influenced the callus induction and the differentiation of the adventitious buds. The results showed that microspore development correlated with the bud diameter and morphological characteristics during development. The microspore development stage, pretreatment at low temperature (4 A degrees C), basic medium, plant growth regulators and genotype all affect hardy kiwifruit anther callus induction, and the type and concentration of plant growth regulators significantly affect the regeneration of adventitious buds. The ploidy of 119 regenerated plants was verified using flow cytometry. The results showed that the ploidy of the plantlets obtained from the vitro anther culture of hardy kiwifruit was complex, being 3.36% haploid, 56.3% tetraploid, 4.20% hexaploid, and 36.13% octoploid.

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