Agrobacterium tumefaciens-mediated transformation as an efficient tool for insertional mutagenesis of Kabatiella zeae
文献类型： 外文期刊
第一作者： Sun, Jiaying
作者： Sun, Jiaying;Xu, Ruidi;Xiao, Shuqin;Lu, Yuanyuan;Xue, Chunsheng;Zhang, Qifeng
作者机构：
关键词： Agrobacterium tumefaciens-mediated transformation (ATMT); Kabatiella zeae; Green fluorescence protein (GFP)
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2018 年 149 卷
页码：
收录情况： SCI
摘要： Agrobacterium tumefaciens-mediated transformation (ATMT) has been widely used in filamentous fungi. In this study, an efficient Agrobacterium tumefaciens-mediated transformation approach was developed for the plant pathogenic fungus, Kabatiella zeae, the causative pathogen of eyespot in maize. Five parameters were selected to optimize efficiencies of transformation. A. tumefaciens concentration, conidia concentration of K. zeae and mixing ratio of A. tumefaciens and K. zeae were found to exert a significant influence on all parameters. Transformants emitted green fluorescence under fluorescence microscopy. The presence of mitotically stable hygromycin resistance gene (hph) integration in the genome was confirmed by PCR. Up to 148 transformants per 10(7) conidia could be obtained under optimal conditions. In this way, ATMT approach is an efficient tool for insertional mutagenesis of K. zeae.
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