Agrobacterium tumefaciens-mediated transformation as an efficient tool for insertional mutagenesis of Kabatiella zeae
文献类型： 外文期刊
第一作者： Sun, Jiaying
作者： Sun, Jiaying;Xu, Ruidi;Xiao, Shuqin;Lu, Yuanyuan;Xue, Chunsheng;Zhang, Qifeng
作者机构：
关键词： Agrobacterium tumefaciens-mediated transformation (ATMT); Kabatiella zeae; Green fluorescence protein (GFP)
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2018 年 149 卷
页码：
收录情况： SCI
摘要： Agrobacterium tumefaciens-mediated transformation (ATMT) has been widely used in filamentous fungi. In this study, an efficient Agrobacterium tumefaciens-mediated transformation approach was developed for the plant pathogenic fungus, Kabatiella zeae, the causative pathogen of eyespot in maize. Five parameters were selected to optimize efficiencies of transformation. A. tumefaciens concentration, conidia concentration of K. zeae and mixing ratio of A. tumefaciens and K. zeae were found to exert a significant influence on all parameters. Transformants emitted green fluorescence under fluorescence microscopy. The presence of mitotically stable hygromycin resistance gene (hph) integration in the genome was confirmed by PCR. Up to 148 transformants per 10(7) conidia could be obtained under optimal conditions. In this way, ATMT approach is an efficient tool for insertional mutagenesis of K. zeae.
分类号：
- 相关文献
作者其他论文 更多>>
-
Post-anthesis water use and biomass accumulation in winter wheat under subsoiling and microsprinkler irrigation
作者：Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Qifeng;Gao, Zile;Wu, Guanghui;Wang, Kai
关键词：Microsprinkler irrigation; Winter wheat; Water consumption characteristics; Post-anthesis biomass accumulation; Grain-filling characteristics; North China Plain
-
Ultrasound-induced modifications in structure, antioxidant activity and functionality of whey protein isolate-propolis polyphenol conjugates
作者：Li, Shanshan;Peng, Shiqin;Wei, Lin;Li, Jingyan;Lu, Yuanyuan;Hu, Fuliang;Ma, Xiaobin;Peng, Shiqin
关键词：Whey protein isolate; Polyphenol; Free radical conjugation; Ultrasound; Structure; Functional property; Antioxidant activity
-
Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages
作者：Sun, Jiaying;Li, Xuefeng;Chen, Chun;Huang, Yee;Xu, Xiangfei;Cui, Xuemei;Hao, Fangjiao;Ji, Quanan;Bao, Guolian;Liu, Yan
关键词：Pasteurella multocida; Outer membrane vesicles; Proteomics; Inflammatory response
-
Effects of chitosan grafted with gallic acid treatment on the postharvest physiology and biochemistry of Saimaiti apricots
作者：Han, Yaru;Liu, Yidong;Sun, Jiaying;Chen, Jiluan;Li, Ziqin;Song, Fangyuan;Han, Yaru;Liu, Yidong;Sun, Jiaying;Chen, Jiluan;Han, Yaru;Liu, Yidong;Sun, Jiaying;Chen, Jiluan
关键词：Chitosan; Gallic acid; Apricot; Volatile compounds; Amino acids; Fatty acids
-
Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles
作者：Huang, Yee;Sun, Jiaying;Cui, Xuemei;Li, Xuefeng;Hu, Zizhe;Ji, Quanan;Bao, Guolian;Liu, Yan;Sun, Jiaying
关键词：Bordetella bronchiseptica; dendritic cells; outer membrane vesicle; subunit vaccine; transcriptomic analysis
-
Potential reinforcing properties of silane-modified rapeseed straw fibers in friction composites: Fractal dimension and circularity characterise powder flowability
作者：Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Zhang, Dexue;Ren, Dongmei;Zhang, Qifeng;Ma, Yunhai;Zhang, Dexue;Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Chen, Mingjiang
关键词：Rapeseed straw fiber; Silane modification; Powder flowability; Fractal dimension; Morphological shape; Friction composites
-
Evaluation of tribological properties of silane-treated rapeseed straw fiber reinforced friction polymer composites prepared by wet granulation
作者：Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Zhang, Cuiying;Zhang, Qifeng;Ma, Yunhai;Li, Lekai;Cong, Xu;Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Zhang, Qifeng;Qi, Zicheng;Yao, Yumei;Zhang, Qifeng;Ma, Yunhai;Li, Lekai;Cong, Xu;Qi, Zicheng
关键词：friction composite; granulation; rapeseed straw fiber; tribological property; wear mechanism
