The E2 glycoprotein is necessary but not sufficient for the adaptation of classical swine fever virus lapinized vaccine C-strain to the rabbit

文献类型： 外文期刊

第一作者： Li, Yongfeng

作者： Li, Yongfeng;Xie, Libao;Zhang, Lingkai;Wang, Xiao;Li, Chao;Han, Yuying;Hu, Shouping;Sun, Yuan;Li, Su;Luo, Yuzi;Qiu, Hua-Ji;Liu, Lihong;Munir, Muhammad

作者机构：

关键词： Classical swine fever virus; C-strain; E2 protein; Adaptation; Rabbit

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2018 年 519 卷

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV) C-strain was developed through hundreds of passages of a highly virulent CSFV in rabbits. To investigate the molecular basis for the adaptation of C-strain to the rabbit (ACR), a panel of chimeric viruses with the exchange of glycoproteins E-rns, E1, and/or E2 between C-strain and the highly virulent Shimen strain and a number of mutant viruses with different amino acid substitutions in E2 protein were generated and evaluated in rabbits. Our results demonstrate that Shimen-based chimeras expressing E-rns-E1-E2, E-rns-E2 or E1-E2 but not E-rns-E1, E-rns, E1, or E2 of C-strain can replicate in rabbits, indicating that E2 in combination with either E(rns)or E1 confers the ACR. Notably, E2 and the amino acids P108 and T109 in Domain I of E2 are critical in ACR. Collectively, our data indicate that E2 is crucial in mediating the ACR, which requires synergistic contribution of E(rns)or E1.

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