Evaluation of the immune response afforded by a subunit vaccine candidate against bluetongue virus in mice and sheep

文献类型： 外文期刊

第一作者： Mohamed, Darien Kheder Ali

作者： Mohamed, Darien Kheder Ali;Du, Junzheng;Gao, Shandian;Tian, Zhancheng;Zhang, Guorui;Huang, Dexuan;Du, Rongsheng;Kang, Biao;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Yin, Hong

作者机构：

关键词： Bluetongue virus; Baculovirus expression; E. coli expression; BTV subunit vaccine; Immune response

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2018 年 219 卷

页码：

收录情况： SCI

摘要： Bluetongue virus (BTV), a vector-borne pathogen, is the causative agent of bluetongue disease in ruminants. In view of the recent emergence of BTV in regions previously known to be free from the disease and/or specific serotypes or strains, optimization of the currently available vaccination strategies to control the spread of vector borne bluetongue is crucial. The main objective of the current study was to develop a subunit vaccine candidate targeting BTV-16, a strain previously isolated in China from sheep with obvious clinical signs. To this end, five polyhistidine-tagged recombinant proteins (BTV-16 VP2, VP3, VP7, NS2 and a truncated version of VP5 (VP54-lamino acids) were expressed using the baculovirus or Escherichia coli expression system for characterization of protective activity. To determine ovine and murine immune responses to the five proteins, sheep and mice were immunized twice at 4- and 2-week intervals, respectively, with one of two different protein combinations in Montanide (TM) ISA201 VG adjuvant or placebo. Data from the competitive enzyme linked immunosorbent assay revealed significantly higher antibody titers in immunized than control animals. Expressed VP5 and NS2 induced a protein-specific humoral response. Interestingly, a serum neutralization test against the BTV-1 serotype showed promising cross-serotype immune response by the vaccine. Based on the collective data, we suggest that these recombinant purified proteins present promising candidates for the design of effective novel vaccines against BTV.

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