Identification of Cucurbit Aphid-Borne Yellows Virus in Cucurbita moschata by Deep Sequencing and Characterization of Virus-Derived Small Interfering RNAs

文献类型： 外文期刊

第一作者： Li, Ding

作者： Li, Ding;Chen, Peng;Hu, Yuanyi

作者机构：

关键词： Cucurbit Aphid-Borne Yellows Virus; Small RNA Deep Sequencing; Virus Derived Small Interfering RNA

期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.708； 五年影响因子：0.739 ）

ISSN： 1556-6560

年卷期： 2018 年 12 卷 2 期

页码：

收录情况： SCI

摘要： Deep sequencing of small RNA populations in cucurbits plants in central China resulted in the identification of the cucurbit aphid-borne yellows virus (CABYV), a member of the genus Polerovirus in the family Luteoviridae. RT-PCR was performed to validate the presence of this virus and the virus-derived small interfering RNAs (vsiRNA) of CABYV in the cucurbits plant were characterized. CABYV-derived siRNAs with length of 21-22 nt were predominated, indicating a possible conserved mechanism of DCL2 and DCL4 participation in the generation of vsiRNAs. A slight preference of C/U towards the 5'-terminal base of CABYV-vsiRNA was observed, suggesting that CABYV-vsiRNA might be loaded by distinct AGO complexes to disturb the gene expression of host plants. The CABYV-vsiRNAs were not evenly distributed along the viral genome, and MP/CP/RTD-coding regions of genome segments were detected in the 5'-terminal regions. Our results might contribute to clarify the host antiviral response induced by CABYV, and give hints on designing antiviral strategies using RNAi against CABYV.

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