Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers

文献类型： 外文期刊

第一作者： Hou, Wanwei

作者： Hou, Wanwei;Yan, Qingbiao;Li, Ping;Sha, Weichao;Tian, Yingying;Liu, Yujiao;Hou, Wanwei;Yan, Qingbiao;Li, Ping;Sha, Weichao;Tian, Yingying;Liu, Yujiao;Zhang, Xiaojuan;Zhang, Xiaojuan

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关键词： faba bean; zt-1; linkage map; SSR; ISSR

期刊名称：AGRONOMY-BASEL （ 影响因子：3.417； 五年影响因子：3.64 ）

ISSN： 2073-4395

年卷期： 2018 年 8 卷 6 期

页码：

收录情况： SCI

摘要： Faba bean (Vicia faba L.), a partially allogamous species, is rich in protein. Condensed tannins limit the use of faba beans as food and feed. Two recessive genes, zt-1 and zt-2, control the zero tannin content in faba bean and promote a white flower phenotype. To determine the inheritance and develop a linkage map for the zt-1 gene in the faba bean germplasm M3290, F-2 and F-3 progenies were derived from the purple flower and high tannin content genotypes Qinghai12 and zt-1 line M3290, respectively. Genetic analysis verified a single recessive gene for zero tannin content and flower colour. In total, 596 SSR markers and 100 ISSR markers were used to test the polymorphisms between the parents and bulks for the contrasting flower colour via Bulked Segregant Analysis (BSA). Subsequently, six SSR markers and seven ISSR markers were used to genotype the entire 413 F-2 population. Linkage analysis showed that the zt-1 gene was closely linked to the SSR markers SSR84 and M78, with genetic distances of 2.9 and 5.8 cM, respectively. The two flanked SSR markers were used to test 34 faba bean genotypes with different flower colours. The closely linked SSR marker SSR84 predicted the zt-1 genotypes with absolute accuracy. The results from the marker-assisted selection (MAS) from this study could provide a solid foundation for further faba bean breeding programmes.

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