Molecular characterization, tissue distribution, localization and mRNA expression of the bucky ball gene in the Dabry's sturgeon (Acipenser dabryanus) during oogenesis

文献类型： 外文期刊

第一作者： Ye, Huan

作者： Ye, Huan;Yue, Huamei;Yang, Xiaoge;Li, Chuangju;Wei, Qiwei;Yue, Huamei;Li, Chuangju;Wei, Qiwei

作者机构：

关键词： Bucky ball; Dabry's sturgeon; Expression; Localization

期刊名称：GENE EXPRESSION PATTERNS （ 影响因子：1.224； 五年影响因子：1.478 ）

ISSN： 1567-133X

年卷期： 2018 年 28 卷

页码：

收录情况： SCI

摘要： In many organisms, germ cells are specified during embryogenesis by the inheritance of maternally deposited RNAs and proteins termed germ plasm. In vertebrates, the bucky ball (buc) gene plays an essential role in the germ plasm aggregation. In this study, the full-length cDNA of buc homologue in Dabry's sturgeon, Adbuc, was isolated and characterized. Multiple sequence alignments showed that the BUVE domain of Buc was highly conserved in vertebrates, despite exhibiting low identities with each other across the whole protein. By quantitative real-time PCR analysis, we found that Adbuc RNAs were only detected in the gonad with a high level in the ovary and a very low level in the testis. During embryogenesis, these RNAs were highly expressed from the unfertilized eggs to blastula, declined dramatically from the gastrula stage, and hardly found after the neurula stage. Moreover, with the development of ovary, the expression level of Adbuc was increasing. By in situ hybridization, the signal of Adbuc was not found in the oogonia, increased slightly in the stage I oocytes, and extremely strong in the stage II oocytes, suggesting that the signal became much stronger with increasing size of oocytes. Additionally, Adbuc co-localized with the mitochondrial cloud. Thus, we conclude that Dabry's sturgeon buc gene might also function in germplasm formation.

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