C/EBP beta Promotes STAT3 Expression and Affects Cell Apoptosis and Proliferation in Porcine Ovarian Granulosa Cells

文献类型： 外文期刊

第一作者： Yuan, Xiaolong

作者： Yuan, Xiaolong;Zhou, Xiaofeng;He, Yingting;Zhong, Yuyi;Zhang, Zhe;Zhang, Hao;Li, Jiaqi;Zhang, Ailing

作者机构：

关键词： ovarian granulosa cells; C/EBP beta; STAT3; cell proliferation; cell apoptosis

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2018 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Previous studies suggest that signal transducer and activator of transcription 3 (STAT3) and CCAAT/enhancer binding protein beta (C/EBP beta) play an essential role in ovarian granulosa cells (GCs) for mammalian follicular development. Several C/EBP beta putative binding sites were previously predicted on the STAT3 promoter in mammals. However, the molecular regulation of C/EBP beta on STAT3 and their effects on cell proliferation and apoptosis remain virtually unexplored in GCs. Using porcine GCs as a model, the 5'-deletion, luciferase report assay, mutation, chromatin immunoprecipitation, Annexin-V/PI staining and EdU assays were applied to investigate the molecular mechanism for C/EBP beta regulating the expression of STAT3 and their effects on the cell proliferation and apoptosis ability. We found that over and interfering with the expression of C/EBP beta significantly increased and decreased the messenger RNA (mRNA) and protein levels of STAT3, respectively. The dual luciferase reporter assay showed that C/EBP beta directly bound at -1397/-1387 of STAT3 to positively regulate the mRNA and protein expressions of STAT3. Both C/EBP beta and STAT3 were observed to inhibit cell apoptosis and promote cell proliferation. Furthermore, C/EBP beta might enhance the antiapoptotic and pro-proliferative effects of STAT3. These results would be of great insight in further exploring the molecular mechanism of C/EBP beta and STAT3 on the function of GCs and the development of ovarian follicles in mammals.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序