Expression of the alpha toxin of Clostridium perfringens in Lactobacillus casei genome and evaluation of its immune effects in mice

文献类型： 外文期刊

第一作者： Song, Baifen

作者： Song, Baifen;Ju, Longzhu;Song, Li;Tang, Lijie;Li, Yijing;Song, Baifen;Cui, Hongyu

作者机构：

关键词： Alpha toxin; Clostridium perfringens; Immune; Lactobacillus casei

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2018 年 118 卷

页码：

收录情况： SCI

摘要： We previously developed a stable and marker-free Lactobacillus casei strain (PP alpha T Delta upp) that contained a chromosomally integrated expression cassette (PP alpha T) that enabled the surface expression of the Clostridium perfringens alpha toxin. To measure immune responses against the alpha toxin, specific-pathogen-free BALB/c mice were inoculated with L. casei PP alpha T Delta upp by oral gavage. Then, specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies and cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM). The results showed that alpha toxin-specific IgA and IgG antibodies and cytokines were markedly increased following immunization. Natural alpha toxin challenge and neutralization tests were performed. The results showed that immunized mice can fully resist 1.5 minimum lethal doses of toxin. These results indicated that the immunized mice can produce not only humoral immunity, but also cellular immunity. These results provide a new pathway for the development of a safe, effective, and food-grade vaccine.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序