Biological characterization and pluripotent identification of ovine amniotic fluid stem cells

文献类型： 外文期刊

第一作者： Pei, Wenhua

作者： Pei, Wenhua;Lu, Tengfei;Ji, Meng;Li, Xiangchen;Guan, Weijun;Wang, Kunfu;Zhang, Shuang;Chen, Fenghao;Li, Lu

作者机构：

关键词： Ovine; Amniotic fluid; Stem cell; Differentiation

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

ISSN： 0920-9069

年卷期： 2018 年 70 卷 3 期

页码：

收录情况： SCI

摘要： Mesenchymal stem cells derived from amniotic fluid have become one of the most potential stem cell source for cell-based therapy for the reason they can be harvested at low cost and without ethical problems. Here, we obtained amniotic fluid stem cells (AFSCs) from ovine amniotic fluid and studied the expansion capacity, cell markers expression, karyotype, and multilineage differentiation ability. In our work, AFSCs were subcultured to passage 62. The cell markers, CD29, CD44, CD73 and OCT4 which analyzed by RT-PCR were positive; CD44, CD73, CD90, CD105, NANOG, OCT4 analyzed by immunofluorescence and flow cytometry were also positive. The growth curves of different passages were all typically sigmoidal. The different passages cells took on a normal karyotype. In addition, AFSCs were successfully induced to differentiate into adipocytes, osteoblasts and chondrocytes. The results suggested that the AFSCs isolated from ovine maintained normal biological characteristics and their multilineage differentiation potential provides many potential applications in cell-based therapies and tissue engineering.

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