文献类型： 外文期刊

第一作者： Xiao Kaizhuan

作者： Xiao Kaizhuan;Xie Hua'an;Zhang Jianfu;Xiao Kaizhuan;Mao Xiaohui;Wang Yingheng;Wang Jinlan;Wei Yidong;Cai Qiuhua;Xie Hua'an;Zhang Jianfu;Xiao Kaizhuan;Mao Xiaohui;Wang Yingheng;Wang Jinlan;Wei Yidong;Cai Qiuhua;Xie Hua'an;Zhang Jianfu;Xiao Kaizhuan;Mao Xiaohui;Wang Yingheng;Wang Jinlan;Wei Yidong;Cai Qiuhua;Xie Hua'an;Zhang Jianfu

作者机构：

关键词： callus; immature embryo; mature seed; japonica rice; RNA sequence; hormone

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2018 年 25 卷 4 期

页码：

收录情况： SCI

摘要： Induced pluripotent cell mass plays a role in genetic transformation mediated by Agrobacterium. Mature seeds are more recalcitrant to the induction of suitable calli than immature embryos in rice, but the exact molecular mechanisms involved remain elusive. In this study, the morphological structure of calli induced from mature seeds and immature embryos were observed under a scanning electron microscope using a paraffin embedded technique. Meanwhile, a total of 2 173 upand down-regulated genes were identified in calli induced from mature seeds and immature embryos by RNA-seq technique and furtherly confirmed by quantitative real-time PCR. The results revealed the remarkable morphological differences in calli induced from mature seeds and immature embryos, and plant hormone signal transduction and hormone biosynthesis pathways, such as abscisic acid, salicylic acid and jasmonic-isoleucine, were found to play roles in somatic embryogenesis. This study provided comprehensive gene expression sets for mature seeds and immature embryos that were served as an important platform resource for further functional studies in plant embryogenesis.

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