Focc alpha 6, a truncated nAChR subunit, positively correlates with spinosad resistance in the western flower thrips, Frankliniella occidentalis (Pergande)

文献类型： 外文期刊

第一作者： Wan, Yanran

作者： Wan, Yanran;Yuan, Guangdi;He, Bingqing;Xu, Baoyun;Xie, Wen;Wang, Shaoli;Zhang, Youjun;Wu, Qingjun;Zhou, Xuguo

作者机构：

关键词： Frankliniella occidentalis; Nicotinic acetylcholine receptor; Diagnostic marker; Truncated alpha 6 subunit; Spinosad resistance

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2018 年 99 卷

页码：

收录情况： SCI

摘要： Nicotinic acetylcholine receptors (nAChRs), a molecular target for spinosyns and neonicotinoids, mediate rapid cholinergic transmission in insect central nervous system by binding acetylcholine. Previous studies have shown that mutations in nAChRs contribute to the high level of resistance to these two classes of insecticides. In this study, we identified nine nAChR subunits from a transcriptome of the western flower thrips, Frankliniella occidentalis, including alpha l-7, beta 1, and beta 2. Exon 4 of alpha 4 and exons 3 and 8 of alpha 6 each have two splicing variants, respectively. In addition, altered or incorrect splicing leads to truncated forms of alpha 3, alpha 5, and alpha 6 subunits. The abundance of every nAChRs in both spinosad susceptible and resistant strains was highest in the 1st instar nymph. Significantly more truncated forms of alpha 6 subunit were detected in spinosad resistant strains, whereas, hardly any full-length form was found in the two highly resistant F. occidentalis strains (resistance ratio > 10(4)-fold). Under laboratory conditions, spinosad resistance was positively correlated with truncated alpha 6 transcripts. The correlation was later confirmed under the field conditions using five field strains. As the molecular target of spinosad, the percentage of truncated nAChR alpha 6 subunits can be used as a diagnostic tool to detect and quantify spinosad resistance in the field.

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