Comparative transcriptome analysis reveals the gene expression profiling in bighead carp (Aristichthys nobilis) in response to acute nitrite toxicity

文献类型： 外文期刊

第一作者： Miao, Ling-Hong

作者： Miao, Ling-Hong;Lin, Yan;Ge, Xian-Ping;Zhou, Qun-Lan;Liu, Bo;Ren, Ming-Chun;Pan, Wen-Jing;Huang, Xin;Ge, Xian-Ping;Liu, Bo;Zhang, Wu-xiao;Liang, Hua-liang;Yu, Han;Ji, Ke;Miao, Ling-Hong;Lin, Yan;Pan, Wen-Jing;Huang, Xin;Ge, Xian-Ping;Zhou, Qun-Lan;Liu, Bo;Ren, Ming-Chun;Zhang, Wu-xiao;Liang, Hua-liang;Yu, Han;Ji, Ke

作者机构：

关键词： Aristichthys nobilis; Expression profiling; Nitrite exposure; Gill; Liver; Pathological mechanisms

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 79 卷

页码：

收录情况： SCI

摘要： Objective: Nitrite exposure induces growth inhibition, metabolic disturbance, oxidative stress, organic damage, and infection-mediated mortality of aquatic organism. This study aimed to investigate the mechanism in responses to acute nitrite toxicity in bighead carp (Aristichthys nobilis, A. nobilis) by RNA-seq analysis. Methods: Bighead carps were exposed to water with high nitrite content (48.63 mg/L) for 72 h, and fish livers and gills were separated for RNA"seq analysis. De novo assembly was performed, and differentially expressed genes (DEGs) between control and nitrite-exposed fishes were identified. Furthermore, enrichment analysis was performed for DEGs to annotate the molecular functions. Results: A total of 406,135 transcripts and 352,730 unigenes were tagged after de novo assembly. Accordingly, 4108 and 928 DEGs were respectively identified in gill and liver in responses to nitrite exposure. Most of these DEGs were up-regulated DEGs. Enrichment analysis showed these DEGs were mainly associated with immune responses and nitrogen metabolism. Conclusions: We suggested that the nitrite toxicity-induced DEGs were probably related to dysregulation of nitrogen metabolism and immune responses in A. nobilis, particularly in gill.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序