Expressional and functional comparisons of two general odorant binding proteins in Agrotis ipsilon

文献类型： 外文期刊

第一作者： Dong, Jian-Zhen

作者： Dong, Jian-Zhen;Li, Jing;Huang, Guang-Zhen;Liu, Jing-Tao;Wang, Qian;Zhang, Yong-Jun;Li, Xian-Chun;Gu, Shao-Hua;Zhou, Jing-Jiang;Liu, Jing-Tao;Li, Xian-Chun;Li, Xian-Chun

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关键词： Agrotis ipsilon; GOBPs; Spatiotemporal expression; Sensilla basiconica; Sensilla trichodea; Plant volatiles; Sex pheromones; Ligand-binding spectrum

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2018 年 98 卷

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收录情况： SCI

摘要： Insect general odorant binding proteins (GOBPs) have been long thought to bind and transport host plant volatiles to the olfactory receptors on the dendrite membrane of the olfactory neurons. Recent studies indicate that they can also bind female sex pheromones. In present study, two GOBP genes, AipsGOBP1 and AipsGOBP2 were cloned from the adult antennae of Agrotis ipsilon. Tissue expression profiles indicated that both of them are antennae-specific and more abundant in the female antennae than in the male antennae. Temporal expression profiles showed that both AipsGOBP1 and AipsGOBP2 began to express in antennae 3 days prior to adult emergence from pupae, and reached their highest expression level 3 and 4 days after adult emergence, respectively. Mating increased their expression in the female antennae but reduced their expression in the male antennae. In situ hybridization and immunolocalization demonstrated that both AipsGOBP1 and AipsGOBP2 are expressed and co-localized in sensilla basiconica and sensilla trichodea of both sexes. AipsGOBP2 exhibited a high binding affinity in vitro with the two major sex pheromone components Z7-12:Ac and Z9-14:Ac and the four plant volatiles cis-3-hexen-l-ol, oleic acid, dibutyl phthalate and jI-caryophyllene with Ki values less than 5 mu M. AipsGOBP1, on the other hand, showed medium binding affinities with the five A. ipsilon sex pheromones and six plant volatiles. AipsGOBP2 also showed a broader ligand-binding spectrum and a greater ligand-binding affinity than AipsGOBP1 with the tested aldehyde and alcohol sex pheromones of Lepidoptera species. Taken together, our results indicate that AipsGOBP2 may play greater roles than AipsGOBP1 does in binding sex pheromones and host plant volatiles.

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