A highly glucose-tolerant GH1 beta-glucosidase with greater conversion rate of soybean isoflavones in monogastric animals

文献类型： 外文期刊

第一作者： Cao, Huifang

作者： Cao, Huifang;Zhang, Yueqi;Shi, Pengjun;Ma, Rui;Yang, Hong;Xia, Wei;Cui, Ying;Luo, Huiying;Bai, Yingguo;Yao, Bin;Xia, Wei

作者机构：

关键词： Glycoside hydrolase (GH) family 1; beta-Glucosidase; Glucose tolerance; Soybean isoflavones; Feed additive

期刊名称：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.346； 五年影响因子：3.426 ）

ISSN： 1367-5435

年卷期： 2018 年 45 卷 6 期

页码：

收录情况： SCI

摘要： In the feed industry, beta-glucosidase has been widely used in the conversion of inactive and bounded soybean isoflavones into active aglycones. However, the conversion is frequently inhibited by the high concentration of intestinal glucose in monogastric animals. In this study, a GH1 beta-glucosidase (AsBG1) with high specific activity, thermostability and glucose tolerance (IC (50) = 800 mM) was identified. It showed great glucose tolerance against substrates with hydrophobic aryl ligands (such as pNPG and soy isoflavones). Using soybean meal as the substrate, AsBG1 exhibited higher hydrolysis efficiency than the GH3 counterpart Bgl3A with or without the presence of glucose in the reaction system. Furthermore, it is the first time to find that the endogenous beta-glucosidase of soybean meal, mostly belonging to GH3, plays a role in the hydrolysis of soybean isoflavones and is highly sensitive to glucose. These findings lead to a conclusion that the GH1 rather than GH3 beta-glucosidase has prosperous application advantages in the conversion of soybean isoflavones in the feed industry.

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