In vivo detection of salicylic acid in sunflower seedlings under salt stress

文献类型： 外文期刊

第一作者： Hu, Ye

作者： Hu, Ye;Zhao, Jing;Li, Haiyang;Wang, Xiaodong;Hou, Peichen;Wang, Cheng;Li, Aixue;Chen, Liping;Hu, Ye;Wang, Xiaodong;Hou, Peichen;Wang, Cheng;Li, Aixue;Chen, Liping;Zhao, Jing

作者机构：

期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

ISSN： 2046-2069

年卷期： 2018 年 8 卷 41 期

页码：

收录情况： SCI

摘要： Salicylic acid (SA) is an important phytohormone. It plays an essential role in regulating many physiological processes of plants. Most of the conventional methods for SA detection are based on in vitro processes. More attention should be paid to develop in vivo methods for SA detection. In this work, Pt nanoflowers and GO were simultaneously electrodeposited and reduced on a Pt wire microelectrode in one step. The Pt nanoflowers/ERGO modified Pt microsensor demonstrated high sensitivity and selectivity for SA. SA could be detected from 100 pM to 1 M with a detection limit of 48.11 pM. Then this microsensor was used to detect SA in the stem of sunflower seedlings under different salt stresses in vivo. The result showed that with the increasing concentration of salt, SA levels decreased. Our result was also confirmed by UPLC-MS and gene expression analysis. To the best of our knowledge, this is the first report of in vivo detection of SA in plants using the Pt nanoflowers/ERGO modified Pt microelectrode. It is foreseeable that our strategy could pave the way for the in vivo detection of phytohormones in plants.

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