Novel amphiphilic polyvinylpyrrolidone functionalized silicone particles as carrier for low-cost lipase immobilization

文献类型： 外文期刊

第一作者： Zhang, Shan

作者： Zhang, Shan;Deng, Qianchun;Li, Ya;Zheng, Mingming;Wan, Chuyun;Zheng, Chang;Tang, Hu;Huang, Fenghong;Shi, Jie

作者机构：

关键词： amphiphilic; polyvinylpyrrolidone; silicone; immobilization

期刊名称：ROYAL SOCIETY OPEN SCIENCE （ 影响因子：2.963； 五年影响因子：3.44 ）

ISSN： 2054-5703

年卷期： 2018 年 5 卷 6 期

页码：

收录情况： SCI

摘要： The high catalytic activity, specificity and stability of immobilized lipase have been attracting great interest. How to reduce the cost of support materials has always been a hot topic in this field. Herein, for the development of low-cost immobilized lipase, we demonstrate an amphiphilic polyvinylpyrrolidone (PVP) grafted on silicone particle (SP) surface materials (SP-PVP) with a rational design based on interfacial activation and solution polymerization. Meanwhile, hydrophilic pristine SP and hydrophobic polystyrenecorded silicone particles (SP-Pst) were also prepared for lipase immobilization. SP-PVP was characterized by X-ray diffraction, scanning electron microscopy; X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and thermogravimetry. Our results indicated that the lipase loading amount on the SP-PVP composites was about 215 mg of protein per gram. In the activity assay, the immobilized lipase SP-PVPSCRL exhibited higher catalysis activity and better thermostability and reusability than SP@CRL and SP-Pst@CRL, The immobilized lipase retained more than 54% of its initial activity after 10 times of re-use and approximately trended to a steady rate in the following cycles. By introducing the interesting amphiphilic polymer to this cheap and easily obtained SP surface, the relative performance of the immobilized lipase can be significantly improved, facilitating interactions between the low-cost support materials and lipase.

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