Elimination of ceftiofur hydrochloride residue in postpartum cows' milk after intramammary infusing at dry-off

文献类型： 外文期刊

第一作者： Kang Ji-jun

作者： Kang Ji-jun;Liu Yi-ming;Zhao Lei-lei;Xu Fei;Chen Xiao-jie;Yan Xing;Li Xiu-bo

作者机构：

关键词： ceftiofur hydrochloride; intramammary infusing; dry cows; milk; residue

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 6 期

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收录情况： SCI

摘要： The purpose of this study was to investigate the residue elimination of ceftiofur hydrochloride in milk of postpartum cows after intramammary infusing at dry-off. An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to detect ceftiofur hydrochloride residue in milk. Through preprocessing, ceftiofur hydrochloride was derivatized into a more stable compound dedfuroyl ceftiofur acetamide (DCA) for further analysis. The linear range of DCA was 0.1 to 50 mu g kg(-1). Average recoveries of DCA were 82.52-105.86%. The intra-day and interday coefficients of variation (CV) were 2.95-9.82 and 6.41-7.43%, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) scores were 0.05 and 0.1 mu g kg(-1), respectively. These parameters showed this method was reliable and valid. Twelve cows were administrated 10 mL ceftiofur hydrochloride by intramammary infusion (corresponding to 500 mg ceftiofur) to each udder after the last milking before the dry-off period. Milk was collected from each udder of cow at 12, 24, 36, 48, 60 and 72 h after calving and was mixed for each time point and each cow, then subjected to UPLC-MS/MS analysis. The results showed, the DCA concentrations in all milk samples were less than LOQ and the maximum residue limit (MRL) 100 mu g kg(-1), which suggested the withdrawal time of ceftiofur hydrochloride intramammary infusion used for preventing and curing mastitis in dry cows was 0 day. The study provided guidance for the clinical applications of ceftiofur hydrochloride intramammary infusion (dry cow).

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