文献类型： 外文期刊
第一作者： Wang, Fang
作者： Wang, Fang;Dong, Qing;Zhou, Benguo;Gao, Zhengliang;Zhao, Xia
作者机构：
关键词： RNA silencing suppressor; Polerovirus; Maize yellow dwarf virus; P0 protein; Co-infiltration assay
期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）
ISSN： 0920-8569
年卷期： 2018 年 54 卷 4 期
页码：
收录情况： SCI
摘要： Maize yellow dwarf virus-RMV2 (MYDV-RMV2) causes dwarfing and yellowing symptoms on leaves in field-grown maize plants in Anhui province in China. Herein, we evaluated the RNA silencing suppressor (RSS) activity of the P0 protein from MYDV-RMV2 by co-infiltration assays using wild-type and GFP-transgenic Nicotiana benthamiana (line 16C). The P0 of MYDV-RMV2 exhibited RSS activity and inhibited RNA silencing both locally and systemically. MYDV-RMV2 P0 acts as an F-box-like motif, and mutations to Ala at positions 67, 68, and 81 in the F-box-like motif (67LPxx81P) abolished the RSS activity of P0. However, MYDV-RMV2 P0 failed to interact with AGO1 from Arabidopsis thaliana. Expressing P0 induced developmental defects. P0 was targeted to both the nuclei and cytoplasm of plant cells. These findings expand our knowledge of the role of polerovirus P0 proteins in RNA silencing.
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