An EMS mutant library for cucumber

文献类型： 外文期刊

第一作者： Lu Xiang-yang

作者： Lu Xiang-yang;Chen Hui-ming;Tian Yun;Chen Chen;Cui Qing-zhi;Liu Xiao-hong;Chen Hui-ming;Huang San-wen;Wang Shen-hao;Huang San-wen;Wang Shen-hao;Chen Chen;Lu Xiang-yang;Chen Hui-ming;Tian Yun;Lu Xiang-yang;Tian Yun

作者机构：

关键词： cucumber; EMS; mutant library; traits

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 7 期

页码：

收录情况： SCI

摘要： Cucumber is an important vegetable crop and a model crop for the study of sex expression in plants. However, the genomic resources and tools for functional genomics studies in cucumber are still limited. In this paper, we conducted ethyl methyl sulfone (EMS) mutagenesis in the northern China ecotype cucumber inbred line 406 to construct a mutant library. We optimized the conditions of EMS mutagenesis on inbred line 406 which included treatment of seeds at 1.5% EMS for 12 h. We obtained a number of mutant lines showing inheritable morphological changes in plant architecture, leaves, floral organs, fruits and other traits through M-1, M-2 and M-3 generations. The F-2 segregating populations were constructed and analyzed. We found that a short fruit mutant and a yellow-green fruit peel mutant were both under the control of a single recessive gene, respectively. These results provide valuable germplasm resources for the improvement of cucumber genetics and functional genomic research.

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